María Elena Lienqueo

María Elena Lienqueo

  • Grado máximo: Doctor en Ciencias de la Ingeniería Mención Química, Universidad de Chile. 1999
  • Jerarquía: Profesor Asociado
  • Área de investigación: Purificación de Bioproductos, Biocombustibles, Biorefineria
  • Email: mlienque@ing.uchile.cl

Publicaciones

  1. Ravanal MC,Sharma S, Gimpel J, Reveco-Urzua FE, Øverland M, Horn SJ, Lienqueo ME (2017) The role of alginate lyases in the enzymatic saccharification of brown macroalgae, Macrocystis pyrifera and Saccharina latissima, Algal Research 26: 287–293.
  2. Pezoa-Conte R, Leyton A, Baccinia A, Ravanal MC, Mäki-Arvela P, Grénman H, Xu C, Willför S, Lienqueo ME, Mikkola JK.(2017) Aqueous extraction of the sulfated polysaccharide ulvan from the green alga Ulva rigida – Kinetics and modeling. BioEnergy Research , DOI: 10.1007/s12155-017-9853-4.
  3. Leyton A, Vergara-Salinas J, Pérez-Correa JR, Lienqueo ME (2017) Purification of phlorotannins from Macrocystis pyrifera using macroporous resins. Food Chemistry (in press) https://doi.org/10.1016/j.foodchem.2017.05.114.
  4. Braia M, Loureiro D, Tubioa G, Lienqueo ME, Romaninia D (2017) Interaction between trypsin and alginate: an ITC and DLS approach to the formation of insoluble complexes. Colloids and Surfaces B: Biointerfaces (in press) http://dx.doi.org/doi:10.1016/j.colsurfb.2017.04.033
  5. Leyton A; R. Pezoa-Conte; P. Mäki-Arvela; J-P Mikkola, M. E Lienqueo (2017) Improvement in carbohydrate and phlorotannin extraction from Macrocystis pyrifera using carbohydrate active enzyme from marine Alternaria sp as pretreatment, Journal of Applied Phycology DOI: 10.1007/s10811-017-1141-3.
  6. L.A. Mejía-Manzano, G. Sandoval, M.E. Lienqueo, P. Moisset,M. Rito-Palomares, J.A. Asenjo (2017) Simulation of mono-PEGylated lysozyme separation in heparin affinity chromatography using a general rate model, Journal of Chemical Technology & Biotechnology (in press) doi: 10.1002/jctb.5309.
  7. L.A. Mejía-Manzano, M.E. Lienqueo,E.J.Escalante-Vázquez,M. Rito-Palomares, J.A. Asenjo (2017) Optimized purification of mono-PEGylated lysozyme by Heparin Affinity Chromatography using Response Surface Methodology Journal of Chemical Technology & Biotechnology (in press) doi: 10.1002/jctb.5269. 
  8. A Leyton, R Pezoa-Conte, A Barriga, A Buschmann, P Mäki-Arvela, J-P Mikkola; Lienqueo ME (2016) Identification and efficient extraction method of phlorotannins from the brown seaweed Macrocystis pyrifera using an orthogonal experimental design, Algal Research 16: 201–208.
  9. MC. Ravanal, R. Pezoa-Conte,S.von Schoultz, J.Hemming, O.Salazar, I. Anugwom, O.Jogunola, P. Mäki-Arvela, S. Willför,J.-P. Mikkola,Lienqueo ME (2016) Comparison of different types of pretreatment and enzymatic saccharification of Macrocystis pyrifera for the production of biofuel, Algal research 13: 141-147.  
  10. Lienqueo ME,Ravanal MC, Pezoa-Conte R, Cortínez V, Martínez L,Niklitschek T,Salazar O,Carmona R, García A,Hyvärinen S, Mäki-Arvela P, Mikkola J-P.  (2016) Second generation bioethanol from Eucalyptus globulus Labill and Nothofagus pumilio: ionic liquid pretreatment boosts the yields, Industrial Crops & Products  (2016), pp. 148-155.  
  11. R. Pezoa-Conte , A. Leyton , I. Anugwom , S. von Schoultz , J. Paranko , P. Mäki-Arvela, S. Willför , M. Muszyński, J. Nowicki , Lienqueo ME, J.-P. Mikkola  (2015) Deconstruction of the green alga Ulva rigida in ionic liquids: closing the mass balance, Algal Research 12 :262–273.
  12. Shene C., Monsalve MT.,Vergara D.,Lienqueo ME., Rubilar M (2016) High pressure homogenization of Nannochloropsis oculata for the extraction of intracellular components: Effect of process conditions and culture age, Eur. J. Lipid Sci. Technol., 118 (4) 631-639.

  13. Mayolo-Deloisa, Karla; Lienqueo, M.E; Andrews, Barbara; Rito-Palomares, Marco; Asenjo, Juan A. (2012). Hydrophobic interaction chromatography for purification of monoPEGylated RNase A. Journal of Chromatography. A Jun 15;1242:11-6.

  14. Mahn, A., Lienqueo, M.E., Quilodrán, C. and Olivera-Nappa, A. (2012) Purification of Transthyretin as Nutritional Biomarker of Selenium Status, J Sep. Sci, 35(22):3184-3189.

  15. Salinas A., Vega M., Lienqueo M.E, Garcia A., Carmona R., Salazar O. (2011). Cloning of novel cellulases from cellulolytic fungi: heterologous expression of a family 5 glycoside hydrolase from Trametes versicolor in Pichia pastoris, Enzyme and Microbial Technology:6-7 49:485-491

  16. F. Acevedo, L. Pizzul, M P Castillo, O Rubilar, ME Lienqueo, G. Tortella, MC Diez (2011) Practical Culture Technique for Enhanced Production of Manganese Peroxidase by Anthracophyllum discolor Sp, Brazilian Archives of Biology and Technology:6 54:1175-1186

  17. Lienqueo ME., Mahn A., Salgado JC, Shene C (2011) “Mathematical Modeling of Chromatographic Elution Curves of Proteins” Chemical Engineering & Technology:1 35:46-57.

  18. R. Carmona, A. Garcia, O. Salazar, Lienqueo M.E. “Liquid biofuels in Chile: The current status”. Energy, 36 (4):2077-2084, (2011).

  19. Lienqueo M.E., C.Shene, A. Quiroga, O.Salazar, J.C. Salgado, J.A Asenjo.(2010) “Experimental validation of mathematical model predictions for the selection of optimal polypeptide tags to assist the purification of recombinant proteins”. Separation Science and Technology 45 (15): 2153 – 2164, (2010)..

  20. R. Pezoa,V Cortinez, S. Hyvärinen, M. Reunanen, J. Hemming, Lienqueo M.E., O.Salazar, R. Carmona, A. Garcia, D. Yu. Murzin, J.-P. Mikkola. “The use of ionic liquids in the pretreatment of forest and agricultural residues for the production of bioethanol”. Cellulose Chem. Technol., 44 (4-6): 165-172, (2010).

  21. Carmona R, Lienqueo M.E., Salazar O, García A . “Bioenergy II: Biological Pretreatment with Fungi as a Tool for Improvement of the Enzymatic Saccharification of Eucalyptus globulus Labill to Obtain Bioethanol”. International Journal of Chemical Reactor Engineering 7 A77, (2009).

  22. Lienqueo M.E., C. Shene, and J.A. Asenjo (2008) “Optimization of Hydrophobic Interaction Chromatography using a Mathematical Model of Elution Curves of a Protein Mixture” J Mol Recognit ;22(2):110-120.

  23. Lienqueo ME., Salazar O., Calado CRC., L.P. Fonseca, J.M.S. Cabral, (2008) “ Influence of trytophan tags on the purification of cutinase, secretad by recombinant S. cerevisiae, using cationic expanded bed adsorption and HIC” Biotechnol Lett 30: 1353-1358.

  24. Lienqueo ME., Salazar O., Henriquez K., Calado CRC., L.P. Fonseca, J.M.S. Cabral, (2007) “ Prediction of retention time of hydrophobic peptide tagged cutinases in HIC” Journal Chromatography A 1154: 460-463.

  25. LienqueoM.E.,A. Mahn, J.C. Salgado, J.A. Asenjo (2007)“Current insights on protein behaviour in hydrophobic interaction chromatography”,Journal Chromatography B 489:53-68.

  26. Mahn A, Lienqueo M.E, Asenjo J.A., (2007) “Optimal operation conditions for protein separation in hydrophobic interaction chromatography”, Journal Chromatography. B 849:263-242.

  27. Lienqueo M.E.,A. Mahn, G. Navarro, J.C. Salgado, T.Perez-Acle, ,I. Rapaport, J.A. Asenjo (2006) “Novel approaches for predicting protein retention time in hydrophobic interaction chromatography”, J Mol Recognit. Jul-Aug;19(4):260-9.


Proyectos

  1. 2016-2018: Fondequip, Incorporación de un Cromatógrafo líquido preparativo para fortalecer una plataforma orientada a la purificación de proteínas y bioproductos, Investigador Responsable.
  2. 2016 – 2018: Apoyo a la Formación de Redes Internacionales entre Centros de Investigación. CONICYT-REDES150014, Algal Biorefinery Netwook: Foods of Norway-ICDB of Chile. Investigador Responsable

  3. 2014 – 2019: Financiamiento Basal de Centros Científicos y Tecnológicos de Excelencia para la creación del CENTRO DE BIOTECNOLocÍA Y BIOINGENIERÍA.Comisión Nacional de lnvestigación Cientifica y Técnológica CONICYT. Investigador Clave.

  4. 2013-2016:Investigador Responsable, CONICYT-AKA Optimization of production of biofuels from macro-algae.

  5. 2012-2014: Investigador Responsable Programa de Cooperación Científica Internacional Chile-México Estudio de docking molecular en proteínas pegiladas para el análisis de su comportamiento en cromatografía de intercambio iónico (IEX) e interacción hidrofóbica (HIC).

  6. 2012-2014: Investigador Responsable Programa de Cooperación Científica Internacional Chile-Argentina CONICYT-MINCYT.”Desarrollo de métodos de purificación de enzimas que combinan la precipitación de afinidad con polímeros de cadena flexible cargados con la cromatografía de interacción hidrofóbica (HIC)”

  7. 2012-2015: Coinvestigador, Fondecyt: 1121088, Improvement of the lignocellulose hydrolysis by use of auxiliary enzymes.

  8. 2010-2011: CONICYT VI-2010 065. “Support for internationalnetworking between Research Centers” Bioproducts Discovery & Development Centre (BDDC) University of Guelph-Biocomsa-U.de Chile. Co-investigador.

  9. 2008-2011: Proyecto FONDECYT N°1080143. “Effects of hydrophobic polypeptide tag fusion on protein purification by hydrophobic interaction chromatography”. Investigador Principal.

  10. 2008-2009: CONICYT-AKA. “Optimal treatment processes of lignocelluloses for bioethanol – consortium: optbio”. Investigador Principal.

  11. 2008-2010: Projecto Domeyko_Energía. “Optimization of the process for lignocellulose treatment toward the obtaining of bioethanol”. Con apoyo de la Universidad de Chile, 2008-2010. Co-Investigador.

  12. 2005-2008: Proyecto FONDEF “Investigación, desarrollo y producción de proteasas criofílicas comerciales y lipasas de origen antártico”. Co-Investigador.

  13. 2003-2006: Proyecto FONDECYT N°1030668. “Study of the effect of surface hydrophobicity amino acidic distribution on protein chromatographic behavior”. Investigador Principal.