Asenjo, Juan A.

Asenjo, Juan A.
  • Grado máximo: Ph.D., (1978), University College, London.
  • Jerarquía: Profesor Titular
  • Área de investigación: Ingeniería Bioquímica y Biotecnología
  • Email: juasenjo@ing.uchile.cl

Publicaciones


  1. Contador, CA; Rodríguez, V; Andrews, BA; and Asenjo, J.A. (2015) Genome-scale reconstruction of Salinispora tropica CNB-440 metabolism to study strain-specific adaptation, Antonie van Leeuwenhoek, aceppted.

  2. Gomez-Escribano, JP; Castro, JF; Razmilic, V; Govind, C; Andrews, BA; Asenjo, JA and Bibb, MJ (2015) “The Streptomyces leeuwenhoekii genome: de novo sequencing and assembly in single contigs of the chromosome, circular plasmid pSLE1 and linear plasmid pSLE2”, BMC Genomics, 16: 485 ISSN: 01471-2164.

  3. Castro, JF; Razmilic, V; Gomez-Escribano, JP; Andrews, BA; Asenjo, JA and Bibb, MJ (2015) “Identification and heterologous expression of the chaxamycin biosynthetic gene cluster from Streptomyces leeuwenhoekii”, Appl Environ Microbiol, 81, 5820–5831.

  4. Parra, LP, Espina, G, Devia, J, Salazar, O, Andrews, BA, Asenjo, JA. (2015) “Identification of lipase encoding genes from Antarctic seawaterbacteria using degenerate primers: Expression of a cold-active lipasewith high specific activity”, Enzyme and Microbial Technology, 68, 56–61. ISSN: 0141-0229.

  5. Rodríguez, V, Lascani, J, Asenjo, JA and Andrews, BA (2015) “Production of Cell-Penetrating Peptides in Escherichia coli Using an Intein-Mediated System”, Applied Biochemistry and Biotechnology, 175, 3025-3037. ISSN: 0273-2289.

  6. Merino, M.P., Andrews, B.A. and Asenjo, J.A. (2015) Stoichiometric Model and Flux Balance Analysis for a Mixed Culture of Leptospirillum ferriphilum and Ferroplasma acidiphilum. Biotechnology Progress (in press). ISBN: 1520-6033.

  7. Campodonico, MA, Andrews, BA, Asenjo, JA, Palsson, BO, Feist AM (2014) Generation of an atlas for commodity chemical production in Escherichia coli and a novel pathway prediction algorithm, GEM-Path, Metabolic Engineering, 25, 140-158, DOI: 10.1016/j.ymben.2014.07.009

  8. Cumsille, P., Asenjo, J.A. and Conca C., (2014) A novel model for biofilm growth and its resolution by using the hybrid immersed interface-level set method. Computers and Mathematics with Applications, 67, 34-51.

  9. Rodriguez V., Asenjo, J.A. and Andrews, B.A., (2014) Design and implementation of a high yield production system for recombinant expression of peptides. Microbial Cell Factories, 13, 65

  10. Gossage, L., Pires, D.E.V., Olivera-Nappa, A., Asenjo, J.A., Bycroft, M., Blundell, T.L., Eisen, T. (2014) An integrated computational approach can classify VHL missense mutations according to risk of clear cell Renal carcinoma. Human Molecular Genetics (in press)

  11. Olivera-Nappa, A ; Reyes, F ; Andrews, BA, Asenjo, J.A., Cold Adaptation, Ca2+ Dependency and Autolytic Stability Are Related Features in a Highly Active Cold-Adapted Trypsin Resistant to Autoproteolysis Engineered for Biotechnological Applications.PLOS ONE 8, 8:UNSP e72355 (2013).

  12. Bull, AT; Asenjo, J.A., Microbiology of hyper-arid environments: recent insights from the Atacama Desert, Chile. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY. 103, 1173-1179 (2013).

  13. Santhanam, R; Rong, XY; Huang, Y; Andrews, BA; Asenjo, J.A.; Goodfellow, M. Streptomyces bullii sp nov., isolated from a hyper-arid Atacama Desert soil. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY. 103; 367-373 (2013).

  14. Acevedo, JP; Rodriguez, V; Saavedra, M; Munoz, M; Salazar, O; Asenjo, J.A.; Andrews, BA.Cloning, expression and decoding of the cold adaptation of a new widely represented thermolabile subtilisin-like protease. JOURNAL OF APPLIED MICROBIOLOGY 114, 352-363 (2013).

  15. Santhanam, R; Okoro, CK; Rong, XY; Huang, Y; Bull, AT; Weon, HY; Andrews, BA; Asenjo, J.A.; Goodfellow, M. Streptomyces atacamensis sp nov., isolated from an extreme hyper-arid soil of the Atacama Desert, Chile INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 62, 2680-2684 (2012).

  16. Sandoval, G; Andrews, BA; Asenjo, J.A. Elution relationships to model affinity chromatography using a general rate model. JOURNAL OF MOLECULAR RECOGNITION 25, 571-579 (2012).

  17. Moisset, P; Vaisman, D; Cintolesi, A; Urrutia, J; Rapaport, I; Andrews, BA; Asenjo, J.A.. Continuous modeling of metabolic networks with gene regulation in yeast and in vivo determination of rate parameters. BIOTECHNOLOGY AND BIOENGINEERING 109, 2325-2339 (2012).

  18. Mayolo-Deloisa, K; Lienqueo, ME; Andrews, B; Rito-Palomares, M; Asenjo, J.A.. Hydrophobic interaction chromatography for purification of monoPEGylated RNase A. JOURNAL OF CHROMATOGRAPHY A 1242, 11-16 (2012).

  19. Aqueous two-phase systems for protein separation: Phase separation and applications. Asenjo, J.A.; Andrews, BA. JOURNAL OF CHROMATOGRAPHY A 1238, 1-10 (2012).

  20. Streptomyces deserti sp nov., isolated from hyper-arid Atacama Desert soil. Santhanam, R; Okoro, CK; Rong, XY; Huang, Y; Bull, AT; Andrews, BA; Asenjo, J.A.; Weon, HY; Goodfellow, M. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY 101, 575-581 (2012).

  21. Aqueous two-phase systems for protein separation: A perspective. Asenjo, J.A.; Andrews, BA; JOURNAL OF CHROMATOGRAPHY A 1218, 8826-8835 (2011).

  22. Atacamycins A-C, 22-membered antitumor macrolactones produced by Streptomyces sp C38. Nachtigall, J;; Kulik, A; Helaly, S; Bull, AT; Goodfellow, M; Asenjo, J.A.; Maier, A; Wiese, J; Imhoff, JF; Sussmuth, RD; Fiedler, HP. JOURNAL OF ANTIBIOTICS 64, 775-780 (2011).

  23. Diverse Metabolic Profiles of a Streptomyces Strain Isolated from a Hyper-arid Environment. Rateb, ME; Houssen, WE; Harrison, WTA; Deng, H; Okoro, CK; Asenjo, J.A.; Andrews, BA; Bull, AT; Goodfellow, M; Ebel, R; Jaspars, M. JOURNAL OF NATURAL PRODUCTS 74, 1965-1971 (2011).

  24. Identification of Transcription Factors Perturbed by the Synthesis of High Levels of a Foreign Protein in Yeast Saccharomyces cerevisiae. Contador, CA; Andrews, BA; Liao, JC; Asenjo, J.A.. BIOTECHNOLOGY PROGRESS 27, 925-936 (2011).

  25. Chaxamycins A-D, Bioactive Ansamycins from a Hyper-arid Desert Streptomyces sp. Rateb, ME; Houssen, WE; Arnold, M; Abdelrahman, MH; Deng, H; Harrison, WTA; Okoro, CK; Asenjo, J.A.; Andrews, BA; Ferguson, G; Bull, AT; Goodfellow, M; Ebel, R; Jaspars, M. JOURNAL OF NATURAL PRODUCTS 74, 1491-1499 (2011).

  26. Mutagenesis Objective Search and Selection Tool (MOSST): an algorithm to predict structure-function related mutations in proteins. Olivera-Nappa, A; Andrews, BA.; Asenjo, J.A.. BMC BIOINFORMATICS 12 AN122 (2011).

  27. Reducing the allowable kinetic space by constructing ensemble of dynamic models with the same steady-state flux. Tan, Y; Rivera, JGL; Contador, CA; Asenjo, J.A.; Liao, JC. METABOLIC ENGINEERING 13, 60-75 (2011).

  28. Stoichiometric Model and Metabolic Flux Analysis for Leptospirillum ferrooxidans.Merino, MP; Andrews, BA; Asenjo, J.A.. BIOTECHNOLOGY AND BIOENGINEERING 107, 696-706 (2010).

  29. Extension of the selection of protein chromatography and the rate model to affinity chromatography. Sandoval, G; Shene, C; Andrews, BA; Asenjo, J.A.. JOURNAL OF MOLECULAR RECOGNITION 23, 609-617 (2010).

  30. Metabolic Flux Analysis of Embryonic Stem Cells Using Three Distinct Differentiation Protocols and Comparison to Gene Expression Patterns. Sepulveda, DE; Andrews, BA; Papoutsakis, ET; Asenjo, J.A.. BIOTECHNOLOGY PROGRESS 26, 1222-1229 (2010).

  31. Non-Homogeneous Biofilm Modeling Applied to Bioleaching Processes. Olivera-Nappa, A; Picioreanu, C; Asenjo, J.A.. BIOTECHNOLOGY AND BIOENGINEERING 106, 660-676 (2010).

  32. Viral vectors for the treatment of alcoholism: Use of metabolic flux analysis for cell cultivation and vector production. Martinez, V; Gerdtzen, ZP; Andrews, BA; Asenjo, J.A.. METABOLIC ENGINEERING 12, 129-137 (2010).

  33. Experimental Validation of the Predictions of a Mathematical Model for Protein Purification and Tag Selection. Lienqueo, ME; Shene, C; Quiroga, A; Salazar, O; Salgado, JC; Asenjo, J.A.. SEPARATION SCIENCE AND TECHNOLOGY 45, 2153-2164 (2010).

  34. Theoretical and Experimental Evaluation of Hydrophobicity of Proteins to Predict their Partitioning Behavior in Aqueous Two Phase Systems: A Review. Andrews, BA; Asenjo, J.A.. SEPARATION SCIENCE AND TECHNOLOGY 45, 2165-2170 (2010).

  35. Asenjo, J.A. and Andrews, B.A. “Protein Purification using Chromatography: Selection of Type, Modelling, and Optimization of Operating Conditions”. Journal of Molecular Recognition. 22, 65-76, (2009).

  36. Hold, C., Andrews, B. and Asenjo, J.A. “A Stoichiometric Model of Acidithiobacillus ferrooxidans ATTC 23270 for Metabolic Flux Analysis”. Biotechnology and Bioengineering, 102, 1448-1459, (2009).

  37. Okoro, C.K., Brown, R, Jones, A. L., Andrews, B., Asenjo, J.A., Goodfellow, M. and Bull, A. T. “Actinomycete diversity in hyper-arid soils of the Atacama Desert, Chile”. Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology, 95, 121-133, (2009).

  38. Lienqueo, M.E., Salgado, J.C., Giaverini, O., Asenjo, J.A. “Computer-aided design to select optimal polypeptide tags to assist the purification of recombinant proteins”. Separation and Purification Technology, 65 86-94, (2009).

  39. Orellana, C.A., Shene, C. and Asenjo, J.A. “Mathematical Modelling of Elution Curves for a Protein Mixture in Ion Exchange Chromatography Applied to High Protein Concentration”. Biotechnology and Bioengineering, ID: 09-103. R1, (2009).

  40. Díaz, H., Andrews, B.A., Hayes, A., Castrillo, J., Oliver, S. and Asenjo, J.A. “Global Gene Expresión in Recombinant and Non-Recombinant Yeast Saccharomyces cerevisiae In Three Different Metabolic States”. (in press, 2009).

  41. Acevedo, J.P., Reyes F., Parra, L., Salazar, O., Andrews, B.A. and Asenjo, J.A. “Cloning of complete genes for novel hydrolytic enzymes from Antarctic sea water bacteria by use of an improved genome walking technique”. Journal of Biotechnology, 133, 277-286, (2008).

  42. Sepulveda, D.E., Andrews, B.A., Asenjo, J.A. and Papoutsakis, E.T. (2008) Comparative Transcriptional Analisys of Emryoid Body versus Two-Dimensional Differentiation of Murine Embryonic Stem Cells. Tissue Engineering. Part A, 14, 1603-1614.

  43. Salgado, J.A, Andrews B.A., Ortuzar, M.F. and Asenjo, J.A. “Prediction of the partitioning behaviour of proteins in aqueous two-phase systems using only their amino acid composition”. Journal of Chromatography A, 1178, 134-144, (2008)

Proyectos


  1. 2014 – 2019: Financiamiento Basal de Centros Científicos y Tecnológicos de Excelencia para la creación del CENTRO DE BIOTECNOLocÍA Y BIOINGENIERÍA.Comisión Nacional de lnvestigación Cientifica y Técnológica CONICYT.

  2. 2007 – 2011: Proyecto MILENIUM – MIDEPLAN. “Instituto Milenio de Estudios Avanzados en Biología Celular y Biotecnología, ICDB”

  3. 2005 – 2008: Proyecto FONDEF. “Research, Development and Production of Commercial Cryophilic Proteases and Lipases of Antarctic Origin”.

  4. 2002 – 2005: Proyecto FONDEF 1031/CONICYT. “Investigación, desarrollo y producción de proteasas y lipasas criofilicas comerciales de fuentes marinas”.

  5. 2000 – 2005: Proyecto MILENIUM – MIDEPLAN. “Instituto Milenio de Estudios Avanzados en Biología Celular y Biotecnología”.

  6. 1997 – 2000: Proyecto CONICYT. “Protein Engineering: Computational Tools, Protein Characterization MIDEPLANand Molecular Biology for the Synthesis of Novel Recombinant and Engineered Proteins”.