Andrews, Bárbara

Andrews, Bárbara
  • Grado máximo: Ph.D. en Biotecnología, (1985), University of London
  • Jerarquía: Profesor Titular
  • Área de investigación: Microbiología Industrial, Biotecnología
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  1. Contador, CA; Rodríguez, V; Andrews, BA; and Asenjo, J.A. (2015) Genome-scale reconstruction of Salinispora tropica CNB-440 metabolism to study strain-specific adaptation, Antonie van Leeuwenhoek, aceppted.

  2. Gomez-Escribano, JP; Castro, JF; Razmilic, V; Govind, C; Andrews, BA; Asenjo, JA and Bibb, MJ (2015) “The Streptomyces leeuwenhoekii genome: de novo sequencing and assembly in single contigs of the chromosome, circular plasmid pSLE1 and linear plasmid pSLE2”, BMC Genomics, 16: 485 ISSN: 01471-2164.

  3. Castro, JF; Razmilic, V; Gomez-Escribano, JP; Andrews, BA; Asenjo, JA and Bibb, MJ (2015) “Identification and heterologous expression of the chaxamycin biosynthetic gene cluster from Streptomyces leeuwenhoekii”, Appl Environ Microbiol, 81, 5820–5831.

  4. Parra, LP, Espina, G, Devia, J, Salazar, O, Andrews, BA, Asenjo, JA. (2015) “Identification of lipase encoding genes from Antarctic seawaterbacteria using degenerate primers: Expression of a cold-active lipasewith high specific activity”, Enzyme and Microbial Technology, 68, 56–61. ISSN: 0141-0229.

  5. Rodríguez, V, Lascani, J, Asenjo, JA and Andrews, BA (2015) “Production of Cell-Penetrating Peptides in Escherichia coli Using an Intein-Mediated System”, Applied Biochemistry and Biotechnology, 175, 3025-3037. ISSN: 0273-2289.

  6. Merino, M.P., Andrews, B.A. and Asenjo, J.A. (2015) Stoichiometric Model and Flux Balance Analysis for a Mixed Culture of Leptospirillum ferriphilum and Ferroplasma acidiphilum. Biotechnology Progress (in press). ISBN: 1520-6033.

  7. Campodonico, MA, Andrews, BA, Asenjo, JA, Palsson, BO, Feist AM (2014) Generation of an atlas for commodity chemical production in Escherichia coli and a novel pathway prediction algorithm, GEM-Path, Metabolic Engineering, 25, 140-158, DOI: 10.1016/j.ymben.2014.07.009

  8. Rodrí­guez, V, Asenjo, JA and Andrews, BA (2014) Design and implementation of a high yield production system for recombinant expression of peptides, Microbial Cell Factories, 13, 65, doi:10.1186/1475-2859-13-65.

  9. Olivera-Nappa, A, Reyes F, Andrews BA, Asenjo JA (2013) Cold Adaptation, Ca2+Dependency and Autolytica Stability Are Related Features in a Highly Active Cold-Adapted Trypsin Resistant to Autoproteolysis Engineered for Biotechnological Applications, PLoS ONE 8(8): e72355. doi:10.1371/journal.pone.0072355.

  10. Santhanam, R; Rong, XY; Huang, Y; Andrews, BA; Asenjo, JA; Goodfellow, M (2013) Streptomyces bullii sp nov., isolated from a hyper-arid Atacama Desert soil, Antonie van Leeuwenhoek, 103, 367-373, DOI: 10.1007/s10482-012-9816-x.

  11. Acevedo, J.P., Rodriguez, V., Saavedra, M., Muñoz, M., Salazar, O., Asenjo J.A. and Andrews, B.A. (2013) Cloning, expression and decoding of the cold adaptation of a new widely represented thermolabile subtilisin-like protease, J. Applied Microbiol., 114, 352–363, DOI:Â 10.1111//jam.12033.

  12. Sandoval, G. Andrews, B.A. and Asenjo, J.A. (2012) Elution relationships to model Affinity Chromatography using a general Rate Model, J. Mol. Recognit., 25, 571–579, DOI:Â 10.1002/jmr.2223.

  13. Moisset, P., Vaisman, D., Cintolesi, A., Urrutia, J., Rapaport, I., Andrews, B.A. and Asenjo, J.A. (2012) Continuous Q1 Modeling of Metabolic Networks with Gene Regulation in Yeast and In Vivo Determination of Rate Parameters. Biotechnology & Bioengineering, 109, 2325 – 2339, DOI:10.1002/bit.24503.

  14. Santhanam, R. Okoro, C. K., Rong, X., Huang, Y., Bull, Weon, H-Y., A. T., Andrews, B. A., Asenjo, J. A., Goodfellow, M. (2012) Streptomyces atacamensis sp.nov., isolated from an extreme hyper-arid soil of the Atacama Desert, Int. J. Systematic and Evolutionary Microbiol., 62, 2680-2684,DOI: 10.1099/ijs.0.038463-0.

  15. Asenjo, J. A., Andrews, B. A., (2012) Aqueous two-phase systems for protein separation: phase separation and applications, J. Chromatography A, 1238, 1-10.

  16. Santhanam, R. Okoro, C. K., Rong, X., Huang, Y., Bull, A. T., Andrews, B. A., Asenjo, J. A., Weon, H-Y., Goodfellow, M. (2012) Streptomyces deserti sp. Nov., isolated from hyper-arid Atacama Desert soil, Antonie van Leeuwenhoek, 101, 575 – 581.

  17. Mayolo-Deloisa, K., Lienqueo, M. E., Andrews, B. A., Rito-Palomares, M., Asenjo, J. A., (2012) Hydrophobic Interaction Chromatography for Purification of monoPEGylated RNase A, J. Chromatography A, 1242, 11-16.

  18. Asenjo, J. A., Andrews, B. A., (2011) Aqueous two-phase systems for protein separation: A perspective, J. Chromatography A, 1218, 8826-8835, DOI: 10.1016/j.chroma.2011.06051.

  19. Rateb, M. E., Houssen, W. E., Harrison, W. T. A., Deng, H., Okoro, C. K., Asenjo, J. A., Andrews, B. A., Bull, A. T., Goodfellow, M., Ebel, R., and Jaspars, M., (2011) Diverse Metabolic Profiles of a Streptomyces Strain Isolated from a Hyper-arid Environment, J. Nat. Prod., 74, 1965–1971, DOI: 10.1021/np200470u.

  20. Rateb, M. E., Houssen, W. E., Arnold, M., Abdelrahman, M. H., Deng, H., Harrison, W. T. A., Okoro, C. K., Asenjo, J. A., Andrews, B. A., Ferguson, G., Bull, A. T., Goodfellow, M., Ebel, R., and Jaspars, M., (2011) Chaxamycins A_D, Bioactive Ansamycins from a Hyper-arid Desert Streptomyces sp., J. Nat. Prod., 74, 1491–1499, DOI: 10.1021/np200320u.

  21. Olivera-Nappa, A., Andrews, B. A and Asenjo, J. A. (2011) Mutagenesis Objective Search and Selection Tool (MOSST): an algorithm to predict structurefunction related mutations in proteins, BMC Bioinformatics, 12:122-144.

  22. Contador, C. A., Andrews, B. A. Liao, J. C. and Asenjo, J: A. (2011) Identification of Transcription Factors perturbed by the Synthesis of High Levels of a Foreign Protein in Yeast Saccharomyces cerevisiae, Biotech. Progress, 27, 925–936

  23. Merino, M. P., Andrews, B. A. and Asenjo, J. A. (2010) Stoichiometric Model and Metabolic Flux Analysis for Leptospirillum ferrooxidans, Biotechnology and Bioengineering, 107, 696-706.

  24. Andrews, B. A. and Asenjo, J. A. (2010) Theoretical and experimental evaluation of hydrophobicity of proteins to predict their partitioning behaviour in aqueous two phase systems: a review, Separation Science and Technology, 45, 2165-2170.

  25. Sandoval, G., Shene, C., Andrews, B. A. and Asenjo, J. A. (2010) Extension of the selection of protein chromatography and the rate model to affinity chromatography, J. Mol. Recognit., 23, 609-617.

  26. Sepulveda, D., Andrews, B. A., Papoutsakis, E. T. and Asenjo, J. A. (2010) Metabolic Flux Analysis of Embryonic Stem Cells Using Three Distinct Differentiation Protocols and Comparison to Gene Expression Patterns, Biotechnol. Prog., 26, 1222-1229

  27. Martinez, V., Gerdtzen, Z. P., Andrews, B. A., and Asenjo, J. A., (2010) Viral vectors for the treatment of alcoholism: Use of metabolic flux analysis for cell cultivation and vector production, Metabolic Engineering, 12, 129-137.

  28. Hold, C., Andrews, B. A., Asenjo, J. A. “A stoichiometric model of Acidothiobacillus ferrooxidans ATCC 23270 for metabolic flux analysis”. Biotechnology and Bioengineering, 102, 1448-1459, (2009).

  29. Asenjo, J. A. and Andrews, B. A. “Protein purification using chromatography: selection of type, modeling and optimization of operating conditions”. J. Mol. Recognit., 22, 65-76, (2009).

  30. Okoro, C. K., Brown, R., Jones, A. l., Andrews, B. A., Asenjo, J. A., Goodfellow, M. and Bull, A. T. “Diversity of culturable actinomycetes in hyper-arid soils of the Atacama Desert, Chile”. Antonie van Leeuwenhoek, 95, 121-133, (2009).

  31. J. P. Acevedo, F. Reyes, L.P. Parra, O. Salazar, B.A. Andrews, J.A. Asenjo, “Screening PCR mediated by CODEHOP and an improved genome walking method to obtain complete new genes of subtilisin-like serine proteases”. J. Biotechnol.,.133, 277–286, (2008).

  32. J.A. Asenjo and B.A. Andrews. “Challenges and Trends in Bioseparations”. J. Chem Tech. Biotecnol., 83,117–120, (2008).

  33. L. Parra, F. Reyes, J. P. Acevedo, O. Salazar, B. A. Andrews, J. A. Asenjo. “Cloning and fusion expression of a cold-active lipase from marine Antarctic origin”. Enzyme Microb Technol., 42 371–377, (2008).

  34. Salgado, J. C., Andrews, B. A., Ortuzar, M. F., and Asenjo, J. A. “Prediction of the partiti oning behaviour of proteins in aqueous two-phase systems using only their amino acid composition”. J. Chromatography A, 1178 134–144, (2008).

  35. Sepulveda, D., Andrews, B. A., Asenjo, J. A. and Papoutsakis, E. T. “Comparative transcriptional analysis of embryoid body versus two-dimensional differentiation of murine embryonic stem cells”. Tissue Engineering, 14, 1603-1614, (2008).

  36. Asenjo, J.A., Ramirez, P., Rapaport, I., Aracena , J., Goles, E. and Andrews, B.A. “A Discrete Mathematical Model Applied to Genetic Regulation and Metabolic Networks”. J. Microbiol. Biotechnol., 17, (2008).


  1. 2014 – 2019: Financiamiento Basal de Centros Científicos y Tecnológicos de Excelencia para la creación del CENTRO DE BIOTECNOLOGIA Y BIOINGENIERÍA.Comisión Nacional de lnvestigación Cientifica y Técnológica CONICYT. Investigador Clave.

  2. 2006 – 2011: Proyecto MILENIUM – MIDEPLAN. “Institute for Cell Dynamics and Biotechnology”. Investigador Clave.

  3. 2002 – 2005: Proyecto FONDEF 1031/CONICYT. “Investigación, desarrollo y producción de proteasas y lipasas criofilicas comerciales de fuentes marinas”. Director Alterno.

  4. 2000 – 2005: Proyecto MILENIUM – MIDEPLAN. “Instituto Milenio de Estudios Avanzados en Biología Celular y Biotecnología”. Investigador Asociado.

  5. Proyecto DID Nº EO69/97. “Phase formation kinetics and modelling of the separation of proteins in aqueous two-phase systems”. Investigador Responsable.

  6. 2000 – 2004: Proyecto FONDECYT Nº 1010142. “Partition of proteins in aqueous two-phase systems: Prediction of behavior using physico-chemical properties”. Investigador Responsable.